Fuente: Nature Methods - Issue - nature.com science feeds
Expuesto el: domingo, 25 de abril de 2010 2:00
Autor: Christian Frøkjær-Jensen
Asunto: Targeted gene deletions in C. elegans using transposon excision
| Targeted gene deletions in C. elegans using transposon excision Nature Methods 7, 451 (2010). doi:10.1038/nmeth.1454 Authors: Christian Frøkjær-Jensen, M Wayne Davis, Gunther Hollopeter, Jon Taylor, Todd W Harris, Paola Nix, Rachel Lofgren, Michael Prestgard-Duke, Michael Bastiani, Donald G Moerman & Erik M Jorgensen We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker. |
